FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2122764
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGC4900.023.23464669
GTATTAG6850.020.650081
CGATCTT31600.020.5257575
GTCCGGT1601.3629578E-619.4062160
CGCGTAT900.005736052519.16662839
CGTATGC2651.1641532E-1018.22637741
CGTCGTC1351.5777587E-417.88885348
GTGTACG1552.2470771E-517.8068331
CGGTCGG2952.910383E-1117.54233768
ATGCCGT3257.2759576E-1216.98458144
TTTCGGA2301.4240504E-716.5003551
CGATCTG62950.016.332365
GATCTTT25400.016.2995646
CTTGTCG1300.002522688615.92304521
TCGTATG2402.3113716E-715.81246840
TATGCCG3301.5643309E-1015.68178643
TGCCGTC3351.9645086E-1015.4477345
TTGTCGG1350.003244296415.33330222
CCGATCT166200.015.2990674
TATACAG10650.015.2256795
Produced by FastQC (version 0.11.2)