FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705431
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTA605.423381E-428.7499147
TAGCGCG700.00133413424.64278610
TGCCGTC1554.089634E-822.25799845
CTAGCGC800.002900051421.5624359
TATGCCG2051.7425918E-920.19506343
TACGGCC1551.0050808E-620.03219819
TATCTCG1952.1555024E-819.4620536
GTCGCAC900.005735628319.1666156
ACGTCGA1301.1814791E-418.57686854
CGATCTT26400.018.5567635
CGTATGC2255.5388227E-918.39994641
GTCGGAC1351.5775395E-417.88883665
CGGGTCG1753.2097287E-617.74280567
CGATCTG54950.017.2656445
TAGGACG2853.2923708E-1016.94731712
ATGCCGT2652.2810127E-916.92447744
GATCTTT20450.016.8703656
CGGGACG1250.00194116916.5599567
AGACGGC4700.016.1488969
GCCGTCT2151.2760938E-616.04646346
Produced by FastQC (version 0.11.2)