FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8741757
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG13800.028.50003443
TGCCGTC14000.024.88931545
TATCTCG16600.024.31657236
GCCGTCT12350.023.18626246
CGTATGC16900.022.86406141
TCGTATG16050.022.78533440
ATGCCGT16700.022.31140344
AGACGGC15200.021.78937569
CCGTCTT14000.021.4393147
TATCGCG3800.020.88184436
CTCGTAT18150.020.71926939
TCTCGTA18250.020.03860738
CGCGTAT2252.582965E-1019.93358639
ATCGCGT4050.019.59283837
CGATCTT117600.019.4210535
TCGCGTA2759.094947E-1218.81841938
CGATCTG204400.018.735455
CCGATCT543300.016.7135334
TTCCGAT560650.016.2701552
GGCGACG13000.015.92309565
Produced by FastQC (version 0.11.2)