FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50242
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGAGCA150.00234241569.061
CCGTGGA150.00234241569.056
CATCTGG150.00234241569.036
TTTGCGG150.00234241569.044
AAGGGGG1050.059.1428669
CCGGGGT309.000103E-657.547
GTGTTAA252.5435365E-455.19999758
GTTTGCG252.5435365E-455.19999743
TGGGTGA252.5435365E-455.19999755
AGCATGG950.054.4736834
GCATGGG850.052.76470635
GTTTGGC200.007318326751.75000442
ATGGGGT200.007318326751.75000437
AGATCCT200.007318326751.75000432
GGTGTTA200.007318326751.75000457
CTGAGAT200.007318326751.75000416
CACTGAT200.007318326751.75000441
CCCGTGG200.007318326751.75000455
ACCTAAA200.007318326751.7500041
TATCTCC200.007318326751.75000442
Produced by FastQC (version 0.11.2)