FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243496
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACTA200.007352106751.7533
GTACTAG200.007352106751.7534
GCCCTAT350.001345728439.4285764
TGTTATC350.001345728439.4285758
ACTGTTC350.001345728439.4285744
ATCGGGG5250.039.4285737
AAGGGGG2200.039.20454469
CATCGGG7100.038.8732436
AGCATCG8500.037.74705534
TGAGCAT12000.037.662532
GAGCATC9850.037.47715833
TCGGGGG3250.037.15384738
TGTGCGT952.1827873E-1036.31579253
GCATCGG7950.035.58490435
GACTATG400.002592334334.535
TATGCCA501.8571239E-434.4999965
AAGTCAC709.694413E-734.4999967
CTGAGCA13900.034.25179731
GTGTGGG1800.032.58333252
CCTGAGC14950.032.07692330
Produced by FastQC (version 0.11.2)