FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161869
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA6650.023.34586525
CTAGTCG7050.023.020
TTGTGCG5350.022.5700917
TGCGCTA5000.022.0810
CCGTCCG7100.021.8661989
ACACCGT8450.021.6390536
CGCTATC4900.021.1224512
GCTATCG5200.020.56730813
ACCGTCC7600.020.4276318
GTCGGCA7650.020.29411923
TCGGCAT7850.020.2165624
AGTCGGC7800.019.90384722
CGATCTT38400.019.3164065
CGATCTG64600.018.9589795
GCGCTAT5700.018.76315911
CACCGTC8300.018.2891567
GATCTTT26850.017.8603366
GTCCGAT8700.017.84482811
CCGATCT174400.017.7643364
AACACCG9500.017.4315785
Produced by FastQC (version 0.11.2)