FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031113
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATG3400.026.38229669
CGAATCG3400.024.35288869
CGATCTG50050.023.712825
TCTGCGC3950.023.5822288
CGATCTT60250.023.0196065
GATCTTT51100.022.887996
ATCTGCG7050.021.531877
CCGATCT162700.021.2687644
TTCCGAT168150.020.7230362
ATAGGCG1507.336257E-720.7004645
TCCGATC172200.020.2757173
ATCTTTT59750.019.5162757
AATCGCG900.0057359719.16662636
GATCTGG21600.019.0073746
CTTCCGA191750.018.3704241
GCGAATC7700.018.3700969
GGCGAAT13000.018.31149968
TCGCACG950.00783656418.15785610
TCTGGCG2601.7716957E-917.2499648
AATCGGG1402.0836027E-417.24996436
Produced by FastQC (version 0.11.2)