FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323279
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3450.10671896411458832No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAT451.00003585E-438.33290545
TGCCGGG553.2521042E-431.36328712
CGTGCAT450.00461808230.66632310
ATCGCAA450.00461808230.66632341
TCCTCCG500.007727254627.59969118
AGAACGG700.001331861824.6425867
TCTATAC750.001988283123.00333
GTATTAT1803.4015102E-1023.0032981
GTATAAT1052.271701E-523.0032981
GTCCGCT800.002895162721.56225865
CGATCCT800.002895162721.56225834
ACAGGGC1056.01061E-419.7140648
GATCTTT4300.019.25566
CCGATCC900.00572606419.16645233
TAATACT1852.6244925E-718.6513254
GTATAGG1301.17640026E-418.5795881
TAAGACA1301.17640026E-418.5795884
TCTGTCC2053.797868E-818.5119888
ATTAGGC950.00781612218.1605013
GTATAGT950.00781612218.1605011