FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2089801
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA1301.8490391E-723.88443433
GGCGTAT750.00199365422.99982549
GTCGTAA1507.336912E-720.70033634
CGTTCGT850.004120333620.29493335
GTATAAG6650.018.676551
TACGACG1702.434388E-618.2645665
CGTAATA1351.5778113E-417.8887522
CCTAGCG1402.0837269E-417.2498683
CGATCTT38900.017.1168355
GTATTAG7000.016.7570151
TAGACGT11700.016.5126954
CTAGACG9000.016.4832083
ATTGGAC4500.016.0998763
CGATCTG75750.015.9860175
GATCTGG23150.015.7968556
GTATATA15700.015.3820491
TAATACT7650.015.3332174
CTAGTAC3201.6989361E-915.0936363
CCGATCT203900.014.8049384
CCGTCAA1400.00413255214.7856029
Produced by FastQC (version 0.11.2)