FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2533134
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC1304.925365E-621.2309278
TTAGCGT850.00412135220.294274
CGATCTT40900.019.4854765
TAATGCG900.0057366919.1664316
ATCGGGG2851.6370905E-1118.15838837
TGTCGCC4650.017.80658310
TAATACT11200.017.5581674
CGATCTG82750.017.2605515
GATCTTT32650.017.0127126
GAACCGT1452.723475E-416.6556246
GCGTTAA1250.00194164116.55979543
GATCTGG25500.016.2357356
ACTGTCG1503.527025E-416.1001198
TTAGTAC6250.016.008123
ATCTGCG8350.015.7007157
TGTACCG2001.1429604E-515.5251155
CCGATCT216450.015.3972034
GCGACGG2756.580558E-815.054955566
TTATACT10100.015.0298154
TTCCGAT227200.014.8660882
Produced by FastQC (version 0.11.2)