FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10817543
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT199350.019.694575
GATCTTT157700.017.9173896
CGATCTG396000.016.126195
CGGTACG3502.1827873E-1115.77140838
GATCTGG123500.015.7276556
GGCGACG23900.015.01253265
CCGATCT1041550.014.8262214
TAGACGT46800.014.669924
ATCGGGG15450.014.51454337
TTCCGAT1078450.014.3893092
GCGACGG13150.014.16728166
TCCGATC1102950.014.1635173
GTATAGA40850.014.1040851
CTTCCGA1127350.013.9885581
CATCGGG19300.013.94298736
ATCTTTT188450.013.913557
TTATACT54450.013.8127174
GGGGTGC55250.013.5503336
CTAGACG34900.013.5430253
GTATAGG31600.013.5380191
Produced by FastQC (version 0.11.2)