FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94442
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG3490.36953897630291604RNA PCR Primer, Index 17 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCTTT150.00234883969.049
GAGCAAC150.00234883969.059
TCAAGCC150.00234883969.061
AGTACGT150.00234883969.021
TAGTCCA150.00234883969.05
GTAGTGA150.00234883969.045
GGTAGTG150.00234883969.044
TAGTACG150.00234883969.020
AGGGGGG1000.062.10000269
GTCGTAT900.061.33333239
TGTTGAA252.555096E-455.22
CTTGAAA2050.053.85365757
CGTGTAA452.9887815E-853.66666857
TCGTATG2500.052.4440
CGGACAC200.00733823951.7500045
TTAGGGG200.00733823951.75000418
AACTTGT200.00733823951.75000421
TAGAACG200.00733823951.7500045
TAGCGGA200.00733823951.75000431
TAGCGAG200.00733823951.75000430
Produced by FastQC (version 0.11.2)