FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61314
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG4410.7192484587533027RNA PCR Primer, Index 17 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG1060.17288058192256253RNA PCR Primer, Index 19 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG680.11090452425220994RNA PCR Primer, Index 17 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATTG150.002344898269.042
GCAACTA150.002344898269.039
CGCATTC150.002344898269.054
CGGTTAT150.002344898269.046
GCTATGC150.002344898269.02
GAATAGG150.002344898269.01
GGGTTAC150.002344898269.059
AACTATT150.002344898269.041
ATGCATA150.002344898269.027
GGTTACA150.002344898269.060
GTCTTGT601.8189894E-1257.549
GCGTATG554.0017767E-1156.45454840
CCGTCTT1850.055.94594647
TCGCGTA501.0859367E-955.238
TATGCCG3000.055.19999743
CGTCTTC1550.053.41935348
CGTATGC2850.053.2631641
TCTTGTG653.6379788E-1253.07692750
CTTGAAA2600.053.07692757
AATCGCG653.6379788E-1253.07692736
Produced by FastQC (version 0.11.2)