FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1929571
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGG500.007736198627.610
CGATCTT35750.023.1608415
TCTTCGC2501.8189894E-1220.78
GCGTAAC1004.3362746E-420.752
CGTAACG1056.025024E-419.71428553
GTATTAT5500.019.4454561
TTCGCCG900.005735797319.16666610
GATCTTT29650.018.3844856
CCGTTAG950.00783632918.15789614
ATCTTCG2952.910383E-1117.5423727
CGATCTG67900.017.4278355
CCGATCT177800.017.1335774
TTCCGAT181850.016.7330232
TCCGATC185900.016.4798283
GATCTGG24300.016.327166
TCTGCGC3401.4551915E-1116.2352948
CTTCCGA192300.016.021061
TAGGACG2804.7366484E-916.01785712
ATCTGCG5600.016.0178577
TCGTATG1958.988678E-615.92307840
Produced by FastQC (version 0.11.2)