FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1404430
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1750.027.641
TGCCGTC2000.027.59999845
TCGTATG1701.6734703E-1024.3529440
TATGCCG2053.6379788E-1223.56097643
ATGCCGT2401.8189894E-1221.562544
CCGTCTT1808.58563E-921.08333447
GCCGTCT1952.1553205E-819.46153846
CGTCCGC1501.6943533E-518.449
TACGCTC1150.001110390218.032
CTAGTAC1402.0829926E-417.253
GTATAAG5000.017.251
CTTACGC1602.9511008E-517.2530
GTATTAT4700.016.8829781
GTGTATA6950.016.8776971
ATAATAC4350.016.6551723
CCGTCGC1452.7229555E-416.65517258
ATAAGAC4150.016.6265073
GTCGTAT1250.00194090116.5639
GTATATA11100.016.4729731
AATCTCG2402.30968E-715.812536
Produced by FastQC (version 0.11.2)