FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194615
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG1704.443791E-922.32337431
CGTCGTA1952.1571395E-819.46140333
CCGTCGT1601.3630652E-619.40611532
ATCTGCG6750.018.401137
GTCGATT950.00783697518.1577739
GATCTGG26100.017.9781176
GTATTAT8250.017.5635151
GCGCGTA1402.0837752E-417.24988220
GACCGTC1804.199974E-617.2498830
GTCGTAA2208.582174E-817.2498834
CGATCTG78500.017.2282985
TACGACG2301.424196E-716.5002615
CTGCGCA3800.016.3431119
ACGCTTA1907.025288E-616.34199324
CGATCTT37850.016.1336775
CGAACTT1503.5271427E-416.09988820
GTATATA11450.015.6680141
CGTGTGG7300.015.59578141
CGCGTAA1554.526691E-415.58053721
TTAAGCG2001.14297745E-515.52489248
Produced by FastQC (version 0.11.2)