FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303220
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT21950.024.0478365
GATCTTT20200.019.6410886
CGGGTCG900.00573482219.16666667
CTATAGT4300.018.4534874
CGATCTG38800.018.1391755
ACGGGTC1150.00111033218.066
CGTTTGC1552.2464115E-517.80645241
ACTATAC2952.910383E-1117.5423723
TCGTCCG1804.197056E-617.2548
CCGATCT102650.017.2079894
ATCTTTT22300.017.0179377
TTCCGAT107500.016.5920932
GTTACGA1250.001940799416.5649
GGCGACC1250.001940799416.5665
TCCGATC109450.016.2964823
GGGGTGC6150.016.2682916
GATCTGG13900.016.1330936
CTTCCGA112850.015.9889241
CGAAAAA6150.015.70731618
CCGAAAA5500.015.68181817
Produced by FastQC (version 0.11.2)