FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2274250
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2200.026.6589841
TATGCCG2350.023.48926543
TGCCGTC1959.349606E-1021.23068245
TCGTATG3050.020.36057340
TTTCGGA2707.2759576E-1219.167011
CGATCTT35000.018.8274785
ATGCCGT2951.8189894E-1218.71178644
CGCGTCC1301.1817094E-418.57684747
TCGAACC950.00783693618.15781838
ACTGTCG1150.001110544718.000328
ATACCGC1402.0830726E-417.2506876
CGATCTG73800.017.2503075
CTGAGCG2109.983378E-716.4288659
CTATACT5050.016.3963324
CTAGTAC4650.016.3228723
GTATCGT1300.00252242915.9233621
GATCTGG24000.015.8131296
GTATGCC4150.015.795114542
CCGATCT194150.015.4422034
GTCGTAT1350.00324443715.33326939
Produced by FastQC (version 0.11.2)