FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129856
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG24391.8782343518974862TruSeq Adapter, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG20281.5617299162148843TruSeq Adapter, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG4920.37888122227698373TruSeq Adapter, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGGGGGGG4680.36039921143420406TruSeq Adapter, Index 11 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG3390.26105840315426315RNA PCR Primer, Index 11 (96% over 28bp)
TCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG3390.26105840315426315TruSeq Adapter, Index 11 (96% over 28bp)
TCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGG2550.19637136520453424TruSeq Adapter, Index 11 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGG2170.16710818137013309RNA PCR Primer, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGAGGGGG2120.16325776244455395TruSeq Adapter, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG1710.1316843272548053TruSeq Adapter, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG1700.1309142434696895TruSeq Adapter, Index 11 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1630.12552365697387877No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAATGGGGGG1320.10165105963528832TruSeq Adapter, Index 11 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGT150.002350832869.08
TACTAAG208.485826E-569.033
CCGGTAG150.002350832869.03
GTCGTAT1450.057.10344739
TAGATAT252.5586877E-455.234
TATCGTA252.5586877E-455.238
GCTTGAA16000.054.55312756
TGTCGTA1400.054.21428338
TCTGCTT15750.054.1047653
CCGTCTT16800.054.00892647
CTTCTGC16150.053.83281751
CTTGAAA16100.053.7857157
TTCTGCT15500.053.64193752
GCCGTCT16950.053.5309746
TGCTTGA16350.053.3853255
TCGTATG17850.053.3445440
CGTCTTC16800.052.9821448
ATGCCGT18250.052.74246244
AGGGGGG4450.052.719169
GTCTTCT16650.052.6306349
Produced by FastQC (version 0.11.2)