FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1696351
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3100.026.70967743
GCCGTCT2650.026.03773746
TGCCGTC3000.024.15000245
TCGTATG3450.024.040
ATGCCGT3400.023.33823644
TTTCGGA2800.022.1785721
CGTATGC3800.021.78947441
CTCGTAT3900.021.23076839
CGATCTT34150.020.4070285
CCGTCTT3500.019.71428547
GGTACGC1108.238928E-418.8181829
TTCGGAA3150.018.619052
GATCTTT23650.018.5264266
GACCGTG950.00783594918.1578967
TCTCGTA4450.017.8314638
CGATCTG67800.017.6061955
ATCTGCG5750.017.47
TCCGCCG1200.001477169717.25000210
TAGCGAT1200.001477169717.25000254
CTAGACG4700.016.8829783
Produced by FastQC (version 0.11.2)