FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881597
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT5250.024.97138444
TATGCCG5200.024.54803543
CGTATGC5650.023.81411641
TCGTATG5350.023.21491240
TGCCGTC5600.022.79460345
CCGTCTT6050.021.09913647
GCCGTCT5750.020.99996446
GGGTCGC1751.4746911E-719.7142566
GTATGCC6850.019.1386542
CTCGTAT6700.019.05220639
CGATCTT57550.019.0034435
GATCTTT41450.018.9773776
TACCGCG3153.6379788E-1217.5240847
CGATCTG108500.016.9478975
CGTCTTC7450.016.67111248
TCTCGTA7550.016.45030238
ATCTTTT44950.016.4251737
GTATAAG7700.016.129841
ACCGCGG3651.8189894E-1216.0687438
CCGATCT280600.015.553254
Produced by FastQC (version 0.11.2)