FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2266004
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT3600.026.83325847
GCCGTCT3650.025.52047346
TATGCCG4200.024.64278643
TGCCGTC3800.024.51308645
ATGCCGT4250.023.5411144
CGTATGC3900.022.99993541
TTTCGGA3250.022.2922421
TCGTATG4550.018.95598840
CGATCTG70900.018.734085
CGATCTT38650.018.6558325
CGTCTTC4650.018.54833248
GCGACGT2053.8276994E-818.51214266
TTCGGAA3750.018.3999482
TCGCGAC950.00783687118.15784361
AGCGTAA3205.456968E-1217.2499510
TACGCTT2208.582174E-817.2499523
GTATAAG6450.017.116231
CCGATCT190150.016.9279024
GATCTTT28650.016.858596
GTATTAT7850.016.7005881
Produced by FastQC (version 0.11.2)