FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379529
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT2150.028.88372247
TATGCCG1800.028.7543
TGCCGTC2050.028.60975545
GCCGTCT2150.027.27907246
TGGACCG1151.6980575E-624.05
ATACCGT1151.6980575E-624.06
CGTCCGG1052.2812083E-522.99999859
CGTATGC2300.022.541
TCGTATG2005.2750693E-1122.42500140
ATGCCGT2550.021.64705844
GCGACGG1657.919334E-820.90909266
AATACCG1004.3354105E-420.75
GGCGACG2655.456968E-1219.52830165
GTATAGG4450.019.382021
CGTACGA900.005734986619.16666668
GTCCGGT1150.001110376618.060
TTCGGAA2156.594564E-817.6511632
CGACGTG1804.1974145E-617.2567
CTAGGAC2208.574716E-817.253
CGATCTT26100.017.0517255
Produced by FastQC (version 0.11.2)