FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286177
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAATAGAATAGGACGTGTGGTTCTATTTTGTTGGTTTCTAGGACCGCCGTAATGATTAATAGGGATAGTCGG17770.13816138836256597No Hit
GTTTATGGTTAAGACTACGACGGTATCTGATCGTCTTCGATCCCCTAACTTTCGTTCCCTGATTAATGAAAACAT15000.11662469473486152No Hit
GATTAATAGGGATAGTCGGGGGCGTCAGTATTCAGCTGTCAGAGGTGAAATTCTTGGATTTGCTGAAGACTAACT13880.10791671752799187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3750.030.3610640
CGTATGC3650.030.24645641
GCCGTCT3350.029.86555346
ATGCCGT3700.029.83772344
TGCCGTC3550.029.15481645
CTCGTAT3950.027.07689539
GTATGCC4450.024.80889142
CCGTCTT4050.022.9999147
CTATGCG750.001993196822.9999089
TCGCGTC750.001993196822.99990830
CTGTGCG1202.4583005E-622.9999089
GCTATCG2950.022.2202513
TATCGGT3000.021.84991515
CGCTAGC800.002899684521.56241432
CCGCTAA1304.9210776E-621.23151239
CGATCTT18900.020.9919825
CGCTATC2800.020.94634612
CGGCGGT4450.020.93250314
TGCGCTA2850.020.57886710
GCGCTAT2850.020.57886711
Produced by FastQC (version 0.11.2)