FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n01_zt14ss10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n01_zt14ss10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749068
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2500.033.1199243
GCCGTCT2700.029.38881546
CCGTCTT3100.028.93541147
ATGCCGT2700.028.1110444
TCGTATG2750.026.3453940
CGTATGC3000.025.29993641
TGCCGTC3250.023.35378845
CTCGTAT3250.022.29225239
TCTGGCG1657.922608E-820.909048
CGAACGG850.004121087520.29406768
CGTCTTC4100.019.35361148
CGTACCC2556.730261E-1118.9411310
TAGACCG3150.018.6195345
CGCCGTT2801.2732926E-1118.48209825
CGGGTCG1501.6947763E-518.39995467
ATAATAC9250.018.2761523
ACTGCGT950.00783615618.157858
ACGGCCG3250.018.04610820
TTGTCGG1552.247173E-517.80640822
CGACCTG1954.596568E-717.69226537
Produced by FastQC (version 0.11.2)