FastQCFastQC Report
Thu 4 Jan 2018
HW37FAFXX_n02_ancestor_barcode_techrep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_ancestor_barcode_techrep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13200147
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAC11645450.0144.850839
CCACCGA3375800.0144.812678
ACACCGA2796200.0144.798728
CACACCG799050.0144.634037
GCACCGA2110350.0144.544468
CTCACCG883200.0144.52877
CCCACCG895000.0144.517477
GACACCG514250.0144.46937
AGCACCG479350.0144.407277
TCACCGA3432150.0144.394098
GTCACCG686900.0144.378657
CGCACCG578650.0144.34267
ACCACCG783700.0144.34217
GCCACCG696150.0144.281597
TCCACCG1026950.0143.791347
GCCTCCT1054400.0143.332477
GGCACCG439200.0143.322397
TACACCG852900.0142.799327
ATCACCG803650.0142.697257
AGCCTCC1061450.0142.375276