FastQCFastQC Report
Thu 4 Jan 2018
HW37FAFXX_n01_ancestor_barcode_techrep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n01_ancestor_barcode_techrep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13200147
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAT12831450.0144.86689
CGCGCAT691850.0144.14117
CTCGCAT1039250.0143.91217
GCGCATA2319900.0143.734718
GCTCGCA192450.0143.664226
GTGCGCA112250.0143.561836
GCCCGCA221250.0143.50826
ACGCATA3141700.0143.433248
GGTCGCA120550.0143.419116
GGCCGCA125000.0143.416786
CACGCAT982900.0143.40847
GCCGCAT641650.0143.397667
CCGCATA3883200.0143.316318
TCGCATA3637950.0143.313678
TCCGCAT1057200.0143.237697
TACGCAT861200.0143.211677
CCCGCAT1190050.0143.200627
TGCGCAT636100.0143.087577
GCGCGCA129700.0143.026386
GATCGCA165500.0143.011526
Produced by FastQC (version 0.11.2)