FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_dgy1706.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_dgy1706.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299684
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATCTCTGTGCTATATCCCTATATAACCTACCCATCCACCTTTCGCT19040.6353358871344482No Hit
GAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTAT16380.5465757264318415No Hit
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG14570.48617877497630835No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA14230.474833491277479No Hit
CTATAATGCAGTCTCTTGATAACTTTTTGCACTGTAGGTCCGTTAAGGTT10370.34603115281429775No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT10220.34102588059422595No Hit
ATATAGAACAGCTAAAGGGTAGTGCTGAAGGAAGCATACGATACCCCGCA8300.2769583961773068No Hit
AGTATACTCCGTCTACTGTACGATACACTTCCGCTCAGGTCCTTGTCCTT7860.26227626433176277No Hit
CTATAGACTATACTAGTATACTCCGTCTACTGTACGATACACTTCCGCTC6640.22156671694184543No Hit
AGTCTATAGTCCGTGGAATTCTAAGTGCCAGCTTTATAATGTCATTCTCC5940.19820877991484362No Hit
GTATTATGCAGCACTTCCAACCTATGGTGTACGATGAAAGTAGGTGTGTA5550.18519507214265693No Hit
CTATATAACCTACCCATCCACCTTTCGCTCCTTGAACTTGCATCTAAACT5420.18085716955192804No Hit
GTACTAGACTACCTTTCATCCTACATAAATAGACGCATATAAGTACGCAT5320.17752032140521348No Hit
GATATAGGTGCGACGTGAACAGTGAGCTGTATGTGCGCAGCTCGCGTTGC5140.17151399474112733No Hit
ATTCTATACCGATGTGGATTGCGCATACTTTGTGAACAGAAAGTGATAGC4880.16283818955966953No Hit
GATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTATTC4680.15616449326624043No Hit
GTTCTATATGCTGCCACTCCTCAATTGGATTAGTCTCATCCTTCAATGCT4680.15616449326624043No Hit
GCATTATAGAGCGCACAAAGGAGAAAAAAAGTAATCTAAGATGCTTTGTT4630.15449606919288317No Hit
ATATATTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCC4360.14548657919675392No Hit
GTTATATAGGGATATAGCACAGAGATATATAGCAAAGAGATACTTTTGAG4360.14548657919675392No Hit
CTAGTATACTCCGTCTACTGTACGATACACTTCCGCTCAGGTCCTTGTCC4350.14515289438208245No Hit
GTCTAGTACCTCCTGTGATATTATCCCATTCCATGCGGGGTATCGTATGC4340.14481920956741098No Hit
GGCCAGGACAACGTATACTCATCAGATAACAGCAATACCTGATCACTACT4300.1434844703087252No Hit
GAGTAATACTAGAGATAAACATAAAAAATGTAGAGGTCGAGTTTAGATGC4290.14315078549405374No Hit
CAAATATACTATCTGTTTCAGGGAAAACTCCCAGGTTCGGATGTTCAAAA4240.14148236142069648No Hit
AGACTATATATTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAA4160.13881288290332483No Hit
ATATAACCTACCCATCCACCTTTCGCTCCTTGAACTTGCATCTAAACTCG4150.1384791980886534No Hit
CCTATATCTGCGTGTTGCCTGTATATATATATACATGAGAAGAACGGCAT4070.13580971957128174No Hit
GAGTATACTAGTATAGTCTATAGTCCGTGGAATTCTAAGTGCCAGCTTTA4010.13380761068325303No Hit
CTATATACTACGTATAGGAAATGTTTACATTTTCGTATTGTTTTCGATTC4000.1334739258685816No Hit
GTATATAGAGACAAAATAGAAGAAACCGTTCATAATTTTCTGACCAATGA3960.13213918660989576No Hit
GACTAATGTTGTGGGAAATTGGAGCGATAAGCGTGCTTCTGCCGTGGCCA3900.13013707772186703No Hit
GGTTATATAGGGATATAGCACAGAGATATATAGCAAAGAGATACTTTTGA3810.12713391438982394No Hit
GCTATAGATTGCATAGAAGAGTTAGCTACTCAATGCTTTTTGTCAAAGCT3570.11912547883770905No Hit
ATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAATGCGTACT3560.11879179402303759No Hit
ATACTAGTATACTCCGTCTACTGTACGATACACTTCCGCTCAGGTCCTTG3530.11779073957902324No Hit
ATTATATTCTATACCGATGTGGATTGCGCATACTTTGTGAACAGAAAGTG3470.1157886306909945No Hit
GTATAATAATATATAGTCTAGCGCTTTACGGAAGACAATGTATGTATTTC3380.11278546735895142No Hit
GTATTATAGCTCATGAATGTGGCTCTCTTGATTGCTGTTCCGTTATGTGT3300.11011598884157979No Hit
ATATACTACGTATAGGAAATGTTTACATTTTCGTATTGTTTTCGATTCAC3270.10911493439756544No Hit
TACTAGTATAGTCTATAGTCCGTGGAATTCTAAGTGCCAGCTTTATAATG3220.10744651032420817No Hit
GGTCTGTAGTAAGGAGAATGACATTATAAAGCTGGCACTTAGAATTCCAC3210.10711282550953671No Hit
ATCATATACTAAAGGAACTAATTCAATCTCAGGGAACCGTAAAGGTTTAT3210.10711282550953671No Hit
GCTTAATACAAGCAAGCAGTCTCTCGCCATTCATATTTCAGTTATTTTCC3190.10644545588019379No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3190.10644545588019379No Hit
ATCCTACCCCTTGCGCTAAAGAAGTATATGTGCCTACTAACGCTTGTCTT3160.10544440143617943No Hit
GTTATTAGTCCCACCAGCTCCAACGGAAGAAGATGTTATGAAGCTCGTAA3120.10410966217749362No Hit
GGGTAGGATCGATCCACTTGTATATTTGGATGAATTTTTGAGGAATTCTG3110.10377597736282217No Hit
GGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGTTCGGATGTTC3100.10344229254815071No Hit
GTATAGGAACTTCAGAGCGCTTTTGAAAACCAAAAGCGCTCTGAAGACGC3080.10277492291880781No Hit
GCATAATACATTGCTTAATACAAGCAAGCAGTCTCTCGCCATTCATATTT3060.1021075532894649No Hit
GCTATATCCCTATATAACCTACCCATCCACCTTTCGCTCCTTGAACTTGC3040.101440183660122No Hit
GTAGTAAGGAGAATGACATTATAAAGCTGGCACTTAGAATTCCACGGACT3020.10077281403077909No Hit
ATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAATG3010.10043912921610762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAGTC2850.078.801127
GCTGTGC300.001940121472.446188
CTCTGTG5450.061.1472367
ATGCAGT4250.056.2523356
TAATGCA4750.054.906584
ATATCTC6500.052.3929063
CTGTGCT6900.050.3973469
ATCTCTG6850.048.6499945
GAGCGCA1203.6307028E-948.297469
ACTCCCT450.00966089648.297466
CTGTGCG604.957621E-448.297469
TCTGTGC7250.047.964378
ACGACAC1700.046.876945145
CCAGCGC803.7447404E-545.2788668
CAGTCTC5150.045.0151069
AATGCAG5350.044.686435
TAGTCCC2000.043.4677126
CTACCCC1703.8198777E-1142.6154064
GTCCCAC2050.042.4075288
TATGCAG3800.041.942535
Produced by FastQC (version 0.11.2)