FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2549768
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36380.14267964771696875No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG22600.020.2003271
TAGGTAG10900.019.2761885
GCCGACC4857.6470315E-917.927994145
TATACAG30450.017.6073515
CTGATCG7050.017.4703249
CTAGGGG3554.7305475E-516.3271833
CTAGGAC6256.0754246E-1016.229223
TATACGG14800.016.154815
GTATATG30600.016.103261
GTATATA56800.015.94732951
CATATAC32750.015.4858973
GTCCTAC5203.2886783E-715.3290641
TAGGGGT7603.8198777E-1115.2530274
GGGACGG5705.607035E-815.2527289
TCTATAC19600.015.1557493
GTATAAG20400.014.5639771
ATACGGT15000.014.49037556
GTGTATA23600.014.4315221
ACTGGTC11150.014.2951578
GTATAGA21900.014.2282221
Produced by FastQC (version 0.11.2)