FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3240945
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49710.15338118974558346No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG33100.018.3871155
GTCCTAG6800.018.1148951
TAGACAG16450.017.177545
GTATTAG25700.016.634691
TCTATAC24800.016.3603253
ACTCCGC9500.016.0158988
GTATAGA28100.015.4717951
TCCTAGG6601.3169483E-915.3687912
ATACAGG25050.015.3295866
CTACACT15650.015.27764
ACCTCGC5253.6732126E-715.18068
GGACAGT14350.015.1471756
TACACTG18900.014.9508225
CTTACAC13150.014.8762533
CTAATAC29800.014.83095553
TACAGCG12500.014.4907987
CTGCCGC42600.014.457451145
TAGGTAG14050.014.4392285
TATACTG37150.014.2372575
CAGCGTG9700.014.1917999
Produced by FastQC (version 0.11.2)