FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3367976
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49910.14818989208949232No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG25100.021.0759721
TAGGTAG11850.018.9549985
GTCCTAG8400.018.1166731
GTATATA68200.017.0009841
GTATATG36300.016.7691521
CTTACAC14900.016.0475253
TATACCG17500.015.7335225
GTATAGA26600.015.5285781
TCTCGGG10750.015.502892145
TACACCG10900.015.2890945
TATACTG37900.014.9119985
TAATACT52900.014.7927354
ATACCGT23150.014.397726
ACTATGC25200.014.3766058
CATATAC39100.014.2690283
TATATAC70450.014.0902923
CTAATAC28350.014.0569163
GTATTAA37500.013.9136061
TATACGG17250.013.8613425
GGTAGTC14750.013.7547567
Produced by FastQC (version 0.11.2)