FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3815277
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56860.14903242936227173No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC6450.024.71533145
CCGACGC33950.020.276234145
GTATTAG30850.019.4964181
TACACCG13450.018.3160425
GTGTAAG18800.017.3454881
GGTAGTC17350.016.7047967
GTATAGA29750.016.5635661
CTATACT32200.016.4263824
TAGGTAG16800.016.388885
GTATATG44500.015.6330281
GTATATA77100.015.6021911
TCTCGGG12200.015.442476145
TACGGTG20950.015.21771057
TATACGG24350.015.1756115
ACGGTGT20250.015.0279328
TATACTG45800.014.3962995
GTATAGG22700.014.3654271
GTGTATG24300.014.3141921
TAGTCAG15400.014.1148259
TATATAC82850.013.7305453
Produced by FastQC (version 0.11.2)