FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578010
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19320.12243268420352214No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC3551.0225813E-720.409397145
GTACGCG1800.00432502820.1252949
GTATATG19100.018.968651
TAGGTAG5951.4551915E-1118.2649735
GTATTAG13300.016.3444161
TATACTG20850.016.3318945
GCTGCCC5854.4219632E-916.100746145
GGTACGC3605.3304975E-516.1002358
ACTATGC10550.015.1082778
TATATAC37000.015.0771763
TATACAG16850.015.0491815
GTATATA36000.014.8946061
AGACGGC4953.321882E-614.6365776
AGACCGT8008.731149E-1114.4902136
AGCGTCC3558.7378034E-414.2861258
TAGACCG6201.5679689E-714.0227855
GTATAGT15050.013.9624391
ACTGGTC6752.9218427E-813.9535378
TTATACT21850.013.9260784
CTGAGCG3650.001078008213.8947249
Produced by FastQC (version 0.11.2)