FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3408181
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50020.14676450575835026No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC5505.456968E-1219.760502145
TATACAG34300.017.7433455
GTATTAG24600.016.7898731
GTATATA67800.016.5656951
TATACCG15450.015.9441285
GTATAAG25600.015.0018071
GTATAGA28050.014.9831381
ATAGGAC14150.014.8488183
CAGCGTG9800.014.7861219
TATACGG18700.014.7228635
GTGTCCG6451.6087142E-814.6027269
ATACCGT22050.014.457546
TAGGTAG13600.014.3838515
TACGGTG17200.014.3219057
TAGGACA14200.014.2863084
GTGTATA26950.014.2503251
TATACTG38350.014.1692295
TCTATAC25600.014.150783
ATACGGT19550.014.082746
TACAGCG13000.013.9330757
Produced by FastQC (version 0.11.2)