FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4700421
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70200.14934832433094822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG33800.018.6501081
GTATATA71000.017.2467461
GTATAGA34500.017.0115831
TAGGTAG18450.016.4931665
ACTATGC29600.016.3996358
TATTAGG33550.016.1966232
GCCGACC8500.016.195696145
CCTAATA36400.015.7246672
CAGCGTG14350.015.6516789
ACTCCGC13450.015.6216468
CTATGCA25900.015.3855859
CTAATAC39450.014.6924453
TAATACT61800.014.6545534
ATACTAT51900.014.5183446
CTGCCGC58250.014.428695145
GGTAGTC17650.014.3672757
TACTATG35650.014.2262227
TATACCG19900.014.199165
TAGGACA16400.014.1369994
CTATACT33800.013.9330994
Produced by FastQC (version 0.11.2)