FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838234
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25740.1400256985780918No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC3555.456968E-1226.533949145
CTGTGTG6100.021.3793329
GTATATA27750.018.8030051
TCGTGCG3201.940347E-518.1130459
GTATTAG13100.017.149371
CCTGTGT7800.016.7197348
TATACAG16700.016.0522795
GTATAGA13550.016.0450021
GTGTAAG7102.237357E-1015.3105461
GCTGTGC5705.6024874E-815.2530928
CAGCGTG4852.687686E-614.9385959
CCGTCCA5857.703966E-814.8619869
GTATAGG10350.014.70404151
TAAGCAG11900.014.6122055
CCGCGCG3000.0047246814.4904379
CTATGCA10200.014.206319
TATATAC32150.014.1974733
ACAGCGT6652.4017936E-814.1635848
ACTGCTC8752.3646862E-1114.0764248
TATACTG19600.014.0468515
Produced by FastQC (version 0.11.2)