FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1674421
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17470.10433457296581923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC2552.718918E-622.735428145
TCGGTCG2755.2387823E-621.0794269
GCACGCC3401.523993E-619.1807279
GTATATA39400.018.5760141
TATACAG20600.017.939795
GTATAAG12850.017.4817921
ACTATGC14950.017.4486898
TAGACAG8400.017.253025
GTATTAG14050.017.0202141
GTGTATA13300.016.8903031
CTAATAC15500.016.829543
ATACAGG15100.016.7959886
TACAGGC10450.016.6421497
TATACCG9150.016.6307815
CTATGCA14300.016.2149419
TCTAGAC6408.4946805E-1015.85073953
CGGTGGG5052.3559733E-715.785365145
TACTATG17050.015.725047
CCTAATA17400.015.4082722
GTATATG22700.015.3229651
Produced by FastQC (version 0.11.2)