FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2394226
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC4102.18497E-819.43989145
CTATGCA14100.017.9855969
TATACGG16150.017.4971775
TAGGTAG10000.017.3895025
CTGTGCG4205.9896047E-717.251499
GTATTAG19750.017.2456361
GGTAGTC9700.017.1803517
ACTATGC16900.017.1494128
GTATAGA20250.016.8198181
CCTACAC7900.016.509023
GTGTATA19900.016.387321
GTATATA46250.016.13891
TATACAG23800.016.1352185
CTAATAC22600.016.0301463
CAGCGTG7753.6379788E-1215.8936319
CTATACA19300.015.7676834
GTATATG27000.015.5672791
ACGGTGT14500.015.4906488
ATACTAT36300.015.369516
TACTATG20700.015.0513017