FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2595579
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG14600.018.858635
TAGACCG9050.018.4144445
CGGTGGG10800.017.444323145
CTGCCGC28450.017.064636145
ACGGTGT12450.016.8774978
GTCTTAG10750.016.1785931
TATACAG24850.015.453575
TAGCCCG2850.003358432315.2540715
GTATAGG12450.015.133591
GTATTAG20250.015.0301111
CTATACT18400.014.5704064
AGACCGT11600.014.3664426
CATATAC28250.014.3634023
ATACAGT23800.014.3087046
ACTATGC17800.014.2471328
GTATATA46350.014.0711871
CTTAGAC12400.014.0241763
CACACTG12400.014.0239055
GTATAAG19800.013.9077331
TACGGTG13550.013.9031577
Produced by FastQC (version 0.11.2)