FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2053809
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20680.100690960064933No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC17650.020.935555145
GCCGACC3351.3239169E-619.465101145
TATACGG13050.019.4300635
GTATTAG15050.018.7780511
TAGGTAG8900.017.9080945
CATATAC25250.017.2149813
CCATGCG4701.0443182E-716.9555369
TACGGTG12250.016.5591727
GTATAGG10750.016.1780131
GTATAGA18150.015.9700321
TATACCG9700.015.6842685
TACGCCG3254.475381E-415.6038355
ACGGTGT11300.015.3868418
GTCCTAG4251.27605945E-515.3453221
CTGCCGC27550.015.253355145
GTATATG25350.014.8644141
ATACGGT15500.014.4892756
GGACCGT4051.4565655E-414.3103956
GGTAGTC8652.0008883E-1114.2380167
GTCTTAG9700.014.1939831
Produced by FastQC (version 0.11.2)