FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881524
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC6500.028.985882145
GTATTAG22250.019.8687971
GTATAGA23400.018.8923381
TATACAG29550.018.8808735
TAGGTAG10800.018.1146035
CTAATAC25450.017.3672433
GTCCTAG6658.367351E-1116.3471241
GTGTAAG13800.016.2799971
GCTGCCC11150.015.597783145
GTATATA57250.015.3172791
CTGCCGC31900.015.21986145
TAGACAG15300.015.15475
GTACTAG12950.015.1100451
CTAGGAC7704.7293724E-1115.0562933
TAATACT38600.015.0172894
GACTAGG7856.548362E-1114.7685958
ACTGTGC11300.014.7481738
GTGTAGG9350.014.7269811
CCTAATA26150.014.6856452
ACAGTGC12050.014.4315518
Produced by FastQC (version 0.11.2)