FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2506244
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25360.10118727466280218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG29350.023.69815
GTCCTAG6750.021.4706881
ATACAGG22750.020.0632326
GCCGACC4904.1836756E-1019.223495145
TACAGGC13750.018.4419617
TAGGTAG9550.018.2078345
GTATATA60000.017.995121
GTATAAG21450.016.553451
GTATTAG19700.016.5525931
ACAGGCA15650.015.7400578
GAGCGCA6158.580173E-915.3147539
CTAATAC20850.015.2899373
TATATAC61250.015.2595833
GTGTAGG7353.783498E-1014.7884851
TAATACT40850.014.7212654
GTGTAAG11500.014.4927141
CTATACA20900.014.2133584
CTTACAC10800.014.0881723
GTATAAT35200.013.9986451
GTCTTAG12000.013.8888511
Produced by FastQC (version 0.11.2)