FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310158
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23600.10215751476738821No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG10100.018.6513885
CCTATAC10900.017.9471873
GTGTTAG15250.017.5815371
GCCGACC3302.5266998E-517.565619145
TATACAG25900.017.064335
TATACGG17100.016.948185
GTATTAG20550.016.9261
TATATAG30850.015.9702935
GTATAGA17850.015.8325681
CTAGACT7903.6379788E-1215.5912514
CTAGGAC6056.9048838E-915.5685143
ATACAGT24450.015.4093276
CTATACA18200.015.1276464
GTCCTAC5756.225491E-815.1230141
GTATATA51450.015.0703511
TACACCG6802.0063453E-914.916895
TACGGTG14650.014.8368877
GTATATG29300.014.5918171
TATACTG28850.014.5660355
CCTACAC7003.0195224E-914.4906933
Produced by FastQC (version 0.11.2)