FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2815264
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCC10550.018.543825145
GTGTAAG12350.017.6037041
GTATATA52500.017.5304951
GTATAGA21350.016.9715651
GTATTAG18500.016.0606061
GTATAGG14350.015.6552331
GCCGACC5102.6412636E-715.628258145
TATACGG16750.015.5720185
CTAGACT8450.015.43380454
CCTATAC14600.015.3838843
TAGGTAG11300.014.7470985
TAGTCCG10850.014.6906989
CCTAGCC4001.3097966E-414.4906263
ACTGTGC11850.014.0623858
GTATTAC22800.013.9851651
CTATACT20750.013.9668694
CTGCCGC33350.013.905038145
TATAAGG23000.013.8610932
ACCGTGT8401.9099389E-1013.8003528
TAGACAG14300.013.6799625
Produced by FastQC (version 0.11.2)