FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921791
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27590.143563998374433No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG8700.021.6554685
CCTAGGA3457.554627E-821.0036332
TATACGG13250.019.1410485
ACTATGC14300.018.2423158
GTATAGA16200.017.4474071
GTATATG26600.017.4373171
TATACAG22500.017.0689535
CTGCCGC18900.016.870024145
TATACTG25400.016.5465625
GTATTAG18750.016.2341421
TACGGTG10750.016.1776817
ATACGGT13700.015.8677086
ACAGTGC9150.015.8388058
TATATAC47600.015.83214953
TAGGACA7452.7284841E-1115.5624234
GTATATA42850.015.3911791
GCCGACC2850.00335567215.255667145
ACGGTGT11400.015.255278
TATAGGG9550.015.1754012
TACAGCG6251.0590156E-815.07220657
Produced by FastQC (version 0.11.2)