FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3159213
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47070.14899280295440667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC10750.031.679728145
GCTGCCC12200.025.53881145
GTATAGA24100.018.3408131
TAGGTAG11050.017.7043425
GAGCGCG2901.875537E-417.4895389
TAGACAG15750.017.4816075
GTATTAG24250.017.3309361
CAGCGCA10400.016.7207669
CTAGACA14100.015.9301864
TATTAGG23750.015.8641952
TCTAGAC14750.015.7194123
ACTCCGC8300.015.7134928
CTATGCA18050.015.6554569
ACTGTGC11250.015.457428
CTAGGAC8701.8189894E-1214.9910333
GGTAGTC13150.014.8770337
ACAGCGC8801.8189894E-1214.8206818
ACTATGC23000.014.4913328
TAAGAGT16500.014.4913324
ATAATAC40800.014.2071893
Produced by FastQC (version 0.11.2)