FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108391
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30040.14247831640336162No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG22650.018.873525
GTATTAG14600.017.870311
TATACCG11050.017.0482655
CATATAC26000.015.8843943
TCTATAC16500.015.8083923
CCTATAC13550.015.5070013
GTACTAG11300.015.3927161
GTATATA47150.015.3709511
CCGCACG3305.022399E-415.3689069
TATATAC51650.015.2906283
TATACTG24200.015.2694695
GTATAGG15200.015.2576911
GACAGCG6251.0608346E-815.0703097
ACTACAC11150.014.9459013
GTCCTAT9700.014.9431011
ACGGTGT11800.014.7362888
GTGTATG13400.014.6029781
GTATATG26100.014.4392711
CTGCCGC24250.014.341974145
GTGTACG6602.166962E-814.2751891
Produced by FastQC (version 0.11.2)