FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002528
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15050.15012049538766No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC8850.025.383879145
TATACAG12400.022.7920045
CCTAAGG3203.402056E-822.64593
GTCCTAA3809.074938E-920.9803941
GTATATG10350.020.3077681
CTATGCA6300.019.5545569
TAGGTAG3751.8168976E-719.3245035
GTATTAG7650.018.9484251
ACTATGC8050.018.9044028
GCCGACC1950.006858627318.581253145
TAATACT16000.018.5696374
TATACTA16000.018.5696375
TAGGCAG3953.1377022E-718.3460455
TATACCG5158.094503E-1018.292615
ATACAGG7650.017.998316
TCTATAC8150.017.7832833
ACCGTCG4205.9718695E-717.2540198
GTGTACG2550.001594272817.0535831
GTATATA26150.016.906851
ATACTAT15900.016.8633616
Produced by FastQC (version 0.11.2)