FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303865
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15900.12194514002599963No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC2002.2555469E-1036.225323145
ACAGTGC6150.021.2050678
TATACAG13750.020.548855
TCTATAC10700.020.3124873
GTGTAAG6950.018.7685151
GTATATA28850.017.583061
CTATACA11150.017.5434284
CAGTGCA6755.456968E-1217.1734859
CTGTACT8150.016.8896854
CCTAGAG3905.6530753E-616.7187392
TATACTG15200.016.6820765
TCTCGGG3504.1874948E-516.560148145
CCTAGAC3956.3811294E-616.5071093
GTATTAG11000.016.4698451
TAGGTAG4851.492208E-716.4314755
ATACAGT12850.016.3507296
ACGGGTG3655.9988495E-515.87898355
ACTATGC10500.015.8701428
TCCTAAG5503.612331E-815.80680752
GTCTACG3706.723072E-515.6686091
Produced by FastQC (version 0.11.2)