FastQCFastQC Report
Sat 23 Dec 2017
HW37FAFXX_n02_bc_c27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW37FAFXX_n02_bc_c27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464525
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20300.13861149519468768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATGC11000.023.7122768
CTATGCA9200.021.263739
TACAGCG5153.8198777E-1119.6969557
CCTAATA13550.018.716382
CTAATAC13500.018.2489663
TACACGG3252.215469E-517.8354845
GTATAGA13100.017.7023581
TACTATG13100.017.6993357
TCTATAC11600.017.4901353
GTATAAT20400.017.4067761
CATAGGG4256.7722794E-717.0492082
CCTATAC6807.2759576E-1217.0492083
GTATAGG8150.016.8946191
TAATACT22900.016.7700184
GTATATA32950.016.7151641
GGACCGT3153.5234878E-416.1014796
CTATAGA9950.016.0233041
TACACCG5453.2245225E-815.9537595
CCTAGAC4601.5475598E-615.7519863
GTATATG15200.015.7334081
Produced by FastQC (version 0.11.2)